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recombinant human egf  (R&D Systems)


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    Structured Review

    R&D Systems recombinant human egf
    Recombinant Human Egf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1276 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egf/Recombinant+Human+EGF+Protein%2C+CF/pmc13091051-268-9-12
    Average 97 stars, based on 1276 article reviews
    recombinant human egf - by Bioz Stars, 2026-09
    97/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Compositions and method for establishing organoid cultures from cryogenically preserved tissue
    Article Snippet: .. Equipment: Stereo microscope Tissue culture hood Table top centrifuge CO2 incubator/37° C. Dissecting tools Materials: Human fresh biopsies: colon/ileum/duodenum Dispose (Corning 40-235) FBS PBS without Mg2+ and Ca2+ (Thermofisher, 10010-023) Advanced DMEM/F12 Thermofisher, 12634-010) Basement Membrane Matrigel (Corning 354234) HEPES (Thermofisher, 15630-080) Glutamax100× (Thermofisher, 25050-061) Thiazovivin (Stemgent, 04-0017) SB431542 (STEM CELL, 72232) CHIR99021 (STEM CELL, 72304) Y27632 (STEM CELL, 72304) N2 Supplement (Thermofisher, 17502-048) B27 Supplement (Thermofisher, 0080085-SA) Pen/Strep (Thermofisher, 15140-122) N-acetylcysteine (Sigma, A9165-5G) EGF (R&D Systems, 236-EG-200) Nicotinamide (Sigma, N0636) LWRN Conditioned Media 24-well culture plate Growth/Thawing Media for Human Organoids (Enteroids): 1. ..

    Membrane:

    Article Title: Compositions and method for establishing organoid cultures from cryogenically preserved tissue
    Article Snippet: .. Equipment: Stereo microscope Tissue culture hood Table top centrifuge CO2 incubator/37° C. Dissecting tools Materials: Human fresh biopsies: colon/ileum/duodenum Dispose (Corning 40-235) FBS PBS without Mg2+ and Ca2+ (Thermofisher, 10010-023) Advanced DMEM/F12 Thermofisher, 12634-010) Basement Membrane Matrigel (Corning 354234) HEPES (Thermofisher, 15630-080) Glutamax100× (Thermofisher, 25050-061) Thiazovivin (Stemgent, 04-0017) SB431542 (STEM CELL, 72232) CHIR99021 (STEM CELL, 72304) Y27632 (STEM CELL, 72304) N2 Supplement (Thermofisher, 17502-048) B27 Supplement (Thermofisher, 0080085-SA) Pen/Strep (Thermofisher, 15140-122) N-acetylcysteine (Sigma, A9165-5G) EGF (R&D Systems, 236-EG-200) Nicotinamide (Sigma, N0636) LWRN Conditioned Media 24-well culture plate Growth/Thawing Media for Human Organoids (Enteroids): 1. ..

    Article Title: Compositions and method for establishing organoid cultures from cryogenically preserved tissue
    Article Snippet: .. Equipment: Inverted light microscope Tissue culture hood Table top centrifuge CO2 incubator/37° C. Dissecting tools Materials: Human frozen biopsies: colon/ileum Dispase (Corning 40-235) FBS PBS without Mg2+ and Ca2+ (Thermofisher, 10010-023) Advanced DMEM/F12 (Thermofisher, 12634-010) Basement Membrane Matrigel (Corning 354234) HEPES (Thermofisher, 15630-080) Glutamax100× (Thermofisher, 25050-061) Thiazovivin (Stemgent, 04-0017) SB431542 (STEM CELL, 72232) CHIR99021 (STEM CELL, 72052) Y27632 (STEM CELL, 72304) N2 Supplement (Thermofisher, 17502-048) B27 Supplement (Thermofisher, 0080085-SA) Pen/Strep (Thermofisher, 15140-122) N-acetylcysteine (Sigma, A9165-5G) EGF (R&D Systems, 236-EG-200) Nicotinamide (Sigma, N0636) LWRN Conditioned Media 24-well culture plate Freezing Media: 80% DMEM (Thermofisher, 11330-032) 10% FBS 10% DMSO (SIGMA, D8418) Growth/Thawing (Recover) Media for Human Organoids (Enteroids): 3. ..

    other:

    Article Title: Ultra-high field microstructural MRI of living cortical organoids
    Article Snippet: From day 6 to day 25 the medium was changed three times per week with neural medium (1X B27 without vitamin A (Gibco, 12587010), 2 mM L-Glutamine, 100 U/mL penicillin-streptomycin (Gibco, 15140122) in Neurobasal medium (Gibco, 21103049)), supplemented with 20 ng/mL EGF (R&D Systems, 236-EG) and 20 ng/mL FGF2.

    Article Title: A degrader of HER2 and EGFR abolishes p95HER2 and shows robust antitumor efficacy in HER2-positive breast cancer.
    Article Snippet: EGF (Cat # 236-EG-200) was purchased from R&D Systems.

    Article Title: Pulmonary organoid models demonstrate compositionally driven epithelial plasticity and immune polarization
    Article Snippet: Briefly, Advanced DMEM/F12 basal medium (ThermoFisher, 11320033) was filtered and supplemented with the following rat growth factors: FGF10 (50 ng/mL; R&D Systems, 7804-FG), FGF9 (50 ng/mL; R&D Systems, 273-F9), and EGF (50 ng/mL; R&D Systems, 3214-EG)( ).

    Recombinant:

    Article Title: Glioblastoma invasion into different organoid hosts reveals cell-intrinsic and proliferative migratory programs
    Article Snippet: .. Cells were maintained in Neurobasal medium (Gibco, 21103-049) supplemented with B-27 (0.5×; Invitrogen, 17504-044), N -2 (0.5×; Invitrogen, 17502-048), recombinant human bFGF (40 ng/mL; Gibco, PHG0026), and EGF (40 ng/mL; R&D Systems, 236-EG) at 37 °C in a humidified 5% CO 2 incubator. .. Cultures were passaged using TrypLE (Gibco, 12604-013).

    Light Microscopy:

    Article Title: Compositions and method for establishing organoid cultures from cryogenically preserved tissue
    Article Snippet: .. Equipment: Inverted light microscope Tissue culture hood Table top centrifuge CO2 incubator/37° C. Dissecting tools Materials: Human frozen biopsies: colon/ileum Dispase (Corning 40-235) FBS PBS without Mg2+ and Ca2+ (Thermofisher, 10010-023) Advanced DMEM/F12 (Thermofisher, 12634-010) Basement Membrane Matrigel (Corning 354234) HEPES (Thermofisher, 15630-080) Glutamax100× (Thermofisher, 25050-061) Thiazovivin (Stemgent, 04-0017) SB431542 (STEM CELL, 72232) CHIR99021 (STEM CELL, 72052) Y27632 (STEM CELL, 72304) N2 Supplement (Thermofisher, 17502-048) B27 Supplement (Thermofisher, 0080085-SA) Pen/Strep (Thermofisher, 15140-122) N-acetylcysteine (Sigma, A9165-5G) EGF (R&D Systems, 236-EG-200) Nicotinamide (Sigma, N0636) LWRN Conditioned Media 24-well culture plate Freezing Media: 80% DMEM (Thermofisher, 11330-032) 10% FBS 10% DMSO (SIGMA, D8418) Growth/Thawing (Recover) Media for Human Organoids (Enteroids): 3. ..



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    Image Search Results


    ( A ) Representative LysoSensor Yellow/Blue DND-160 fluorescence images showing lysosomal pH in Ctrl and cKO primary RTECs treated with HG + PA, with quantification. ( B ) Calculated lysosomal pH values in indicated group, derived from the LysoSensor Yellow/Blue fluorescence ratio using a calibration curve. ( C ) Measurement of lysosomal phospholipase A 2 (PLA 2 ), phospholipase C (PLC), and phospholipase D (PLD) activities in indicated group. ( D ) Immunoblot analysis of EGFR degradation following EGF stimulation in Ctrl and cKO primary RTECs under HG + PA conditions, with quantification. ( E and F ) Immunoblotting of cathepsin B (CTSB) maturation (E) and LC3 and p62 (F) in HG + PA-treated Ctrl and cKO RTECs, with quantification. ( G ) Cell viability assay of HG + PA-treated Ctrl and cKO RTECs. ( H ) Calculated lysosomal pH measurement in HG + PA-treated Ctrl and cKO RTECs following Forskolin treatment with or without BafA1. ( I and J ) Measurement of lysosomal phospholipase A2 activities (I) and cathepsin activities (J) in HG + PA-treated Ctrl and cKO RTECs following Forskolin treatment with or without BafA1. ( K and L ) Immunofluorescence staining of Nile Red (K) or LipidTOX (L) (red) together with LAMP1 (green) in HG + PA-treated Ctrl and cKO RTECs following Forskolin treatment with or without BafA1, with corresponding quantitative analyses. ( M ) Cell viability analysis of HG + PA-treated Ctrl and cKO RTECs following Forskolin treatment with or without BafA1. Data are presented as mean ± SEM. P values were determined by unpaired two-tailed Student’s t test for (A) to (G) and by one-way ANOVA followed by Tukey’s multiple-comparisons test for (H) to (M). Scale bar: 10 μm in (A), (K), and (L). n = 6 for (A), (K), and (L), n = 4 for (B) and (H), n = 3 for (C) to (F), (I), (J), and (M), n = 5 for (G). * P < 0.05, ** P < 0.01, *** P < 0.001; ns, not significant.

    Journal: Science Advances

    Article Title: Renoprotective effects of tubular glucagon receptor activation mediated by V-ATPase

    doi: 10.1126/sciadv.aeg2534

    Figure Lengend Snippet: ( A ) Representative LysoSensor Yellow/Blue DND-160 fluorescence images showing lysosomal pH in Ctrl and cKO primary RTECs treated with HG + PA, with quantification. ( B ) Calculated lysosomal pH values in indicated group, derived from the LysoSensor Yellow/Blue fluorescence ratio using a calibration curve. ( C ) Measurement of lysosomal phospholipase A 2 (PLA 2 ), phospholipase C (PLC), and phospholipase D (PLD) activities in indicated group. ( D ) Immunoblot analysis of EGFR degradation following EGF stimulation in Ctrl and cKO primary RTECs under HG + PA conditions, with quantification. ( E and F ) Immunoblotting of cathepsin B (CTSB) maturation (E) and LC3 and p62 (F) in HG + PA-treated Ctrl and cKO RTECs, with quantification. ( G ) Cell viability assay of HG + PA-treated Ctrl and cKO RTECs. ( H ) Calculated lysosomal pH measurement in HG + PA-treated Ctrl and cKO RTECs following Forskolin treatment with or without BafA1. ( I and J ) Measurement of lysosomal phospholipase A2 activities (I) and cathepsin activities (J) in HG + PA-treated Ctrl and cKO RTECs following Forskolin treatment with or without BafA1. ( K and L ) Immunofluorescence staining of Nile Red (K) or LipidTOX (L) (red) together with LAMP1 (green) in HG + PA-treated Ctrl and cKO RTECs following Forskolin treatment with or without BafA1, with corresponding quantitative analyses. ( M ) Cell viability analysis of HG + PA-treated Ctrl and cKO RTECs following Forskolin treatment with or without BafA1. Data are presented as mean ± SEM. P values were determined by unpaired two-tailed Student’s t test for (A) to (G) and by one-way ANOVA followed by Tukey’s multiple-comparisons test for (H) to (M). Scale bar: 10 μm in (A), (K), and (L). n = 6 for (A), (K), and (L), n = 4 for (B) and (H), n = 3 for (C) to (F), (I), (J), and (M), n = 5 for (G). * P < 0.05, ** P < 0.01, *** P < 0.001; ns, not significant.

    Article Snippet: RTECs were serum-starved for 4 hours and stimulated with recombinant human EGF (100 ng/ml, MCE HY-P7109) for 0–120 min at 37°C.

    Techniques: Fluorescence, Derivative Assay, Western Blot, Viability Assay, Immunofluorescence, Staining, Two Tailed Test